Coding

Part:BBa_K5471007:Design

Designed by: Michail Diamantakis   Group: iGEM24_Patras-Med   (2024-09-29)


eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was excised from an already existing plasmid backbone, and was then immediately ready for use.


Source

This part is directly taken from "sc AAV miR26a eGFP", and specifically the region from positions 1315 to 2031 (from 5' end)

sc AAV miR26a eGFP was a gift from Joshua Mendell (Addgene plasmid # 21894 ; http://n2t.net/addgene:21894 ; RRID:Addgene_21894)


References

https://www.addgene.org/21894/

Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Kota J, Chivukula RR, O'Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, Mendell JR, Mendell JT. Cell. 2009 Jun 12. 137(6):1005-17. 10.1016/j.cell.2009.04.021 PubMed 19524505